NAD+

02

Each size is tested and released separately and carries its own certificate. All three are set out below: the interactive verification record, the certificate document, and the same information reproduced as text on this page. The panels are not identical across the three current lots, which is why they are presented separately rather than combined.

100 mg

Certificate of analysis - lot J4Q90F, 100 mg

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Lot record - 100 mg

J4Q90F

500 mg

Certificate of analysis - lot 8A0BSG, 500 mg

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Lot record - 500 mg

8A0BSG

1000 mg

Certificate of analysis - lot NAD1000-052026-1, 1000 mg

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Lot record - 1000 mg

NAD1000-052026-1

All figures above are specific to the lots named and are not specifications carried across other lots. The three current lots differ in panel scope, which is precisely why this catalogue states results per lot rather than per product. Read the certificate supplied with your unit.

03

NAD exists as a redox pair. The oxidised form carries a positive charge on the nicotinamide ring and is the electron acceptor. The reduced form has taken on a hydride and is the electron donor. They are two different compounds with different chemistry, different absorbance behaviour and different stability, and they interconvert readily.

They also differ in mass by about two daltons, which on a molecule of 663 is roughly three parts in a thousand. A purity percentage will not distinguish them and neither will a retention time on a coarse method. The mass will: the oxidised form gives a protonated ion near 664 and a doubly protonated ion near 333. All three lots above show exactly that. The peak near 1327 is a dimer adduct of two molecules associating in the source and is expected rather than a contaminant.

There is a second reason this matters beyond identity. In a dehydrogenase assay the reduced form absorbs strongly at 340 nanometres and the oxidised form does not. That difference is the entire basis of the standard spectrophotometric readout, so starting material contaminated with the reduced form raises the baseline before the reaction begins and compresses the signal being measured. On this compound, redox state is not a purity footnote. It is the assay.

04

This catches people out and is worth stating plainly. The oxidised form is labile in alkaline conditions. The reduced form is labile in acidic conditions. There is no single pH at which both are comfortable, so a buffer chosen for one is actively degrading the other, and any assay cycling between the two states is running under conditions that suit only half the reaction.

Two further liabilities apply to the material rather than the assay. This compound is hygroscopic, so a container that has not reached room temperature will draw condensation into the powder on opening. And it is light-sensitive, which is why our units are amber glass rather than clear. That is not a packaging preference; clear glass on this material is a storage decision made against the compound.

05

Panels differ between products, between sizes and between lots. The columns below reflect the three current lots. Nothing is claimed unless it appears on the certificate for the lot supplied.

Endotoxin and microbial detection answer different questions and appear only on the 1000 mg lot. A microbial assay asks whether organisms are present; endotoxin is a bacterial membrane fragment that persists after the organism producing it is gone. Material can pass one and not the other. Where a parameter is material to intended laboratory work, purchasers should verify it independently or order the size released against it.

06

Most of this catalogue consists of compounds whose research application is speculative. This one is not. It has been a standard laboratory reagent for the better part of a century and appears in dehydrogenase assays, coupled enzyme systems, sirtuin and PARP activity work, and any protocol requiring a defined electron acceptor. That is an unglamorous use, it accounts for a substantial share of who buys this compound, and it is the reason grade and redox purity matter here more than anything else on the page.

07

The three units contain the same material and differ in fill. On a hygroscopic, light-sensitive, pH-labile compound the larger size is not automatically the better value: every access is an opportunity for moisture ingress, and a container that outlives the work it was opened for has spent that time degrading rather than waiting. Where small quantities are needed repeatedly, several small units usually give better material at the point of use than one large unit opened repeatedly. Note also that the three sizes are released against separate certificates with different panels, so the applicable testing scope depends on which is ordered.

08

Preparation conditions are the responsibility of the receiving laboratory. Verum provides no protocol, no preparation instruction, and no guidance on use.

09

This page carries no bibliography, for an unusual reason: the biochemistry of this molecule is textbook material rather than a contested literature. Its role as an electron carrier has been established since the early twentieth century and appears in every general biochemistry course. There is nothing here to curate that a researcher does not already have. The literature that is contested concerns administration to people, which is a separate question from what this compound does in a cuvette, and we do not cite it.

10

By placing an order you confirm each of the following:

Confirmation is recorded at checkout against your order. Verum reserves the right to decline or cancel any order.

11

Orders placed before the daily cutoff are dispatched the same day from our United States warehouse. Domestic orders typically arrive within two business days. A tracking reference is issued by email once the consignment leaves the facility. Each unit carries a scannable code linking to the certificate for its own lot.

Not for human or veterinary use. Not for use in diagnostic or therapeutic procedures. This product is a laboratory reagent. Nothing on this page constitutes medical advice, a recommendation for personal use, or a representation that this material is safe or effective for any purpose. No statement here has been evaluated by the U.S. Food and Drug Administration.